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Center for Molecular Medicine

Dedicated to understanding the intricacies of cellular signaling, the Center for Molecular Medicine was built on Caltech's successes at the interface of chemistry and biology. It is focused on determining how complex systems of molecules interact to create the pathways that regulate the lives of cells and allow them to respond to their environments. Rather than just studying a complex biomolecule in isolation, an understanding of how it is part of a larger path of molecules is the purpose of its establishment. The Center was made possible by a generous grant from the Gordon and Betty Moore Foundation. 

If use of the Center instrumentation has contributed significantly to your research, we request that you cite the Center in the acknowledgments section of your publication.  For example: "This work was supported by…, and by the Center for Molecular Medicine at California Institute of Technology."

Instrumentation:

Crellin Room 260 (Bacterial)

Crellin Room 262 (Mammalian)

How to get access to CM2 and use the instruments in CM2?

1. Contact the appropriate caretakers for each instrument you would like to use for the instrument training.

2. Upon completion of training, contact Dina Malounda (malounda@caltech.edu) to get access.


People in the Facility:

  • Faculty members: 
    Dr. Dennis Dougherty
    Dr. Jacqueline Barton
    Dr. Peter Dervan
    Dr. Linda Hsieh-Wilson
    Dr. Shu-ou Shan
    Dr. Alison Ondrus
    Dr. Mikhail Shapiro
     
  • Lab Assistants:
    Please see the table of equipment caretakers

  • Safety Officer
    Dina Malounda malounda@caltech.edu
     

Seminars:

Thursday afternoons
151 Crellin
12:00 to 1:00 p.m.
Upcoming Seminars:

January 31, 2019 - David Romney
February 7, 2019 - Rebekah Silva
February 14, 2019 - Patrycja Kielb
February 21, 2019 - Elliot Mackrell
February 28, 2019 - George Mobbs
March 7, 2019 - Arash Farhadi
March 14, 2019 - Christopher J. Bley

Information on Instrumentation:


For training or troubleshooting, please contact the appropriate caretakers.


Equipment

Caretaker

Contact info

Biosafety cabinet (TC hood)

Alice Hsu

ahhsu@caltech.edu

Chemical fume hood

Dina Malounda

malounda@caltech.edu

Amaxa 4-D Nucleofector

John Thompson

jthompso@caltech.edu

Benchtop centrifuges

Dina Malounda

malounda@caltech.edu

Benchtop water bath

Alice Hsu

ahhsu@caltech.edu

MilliQ water deionizer

Brendan O'Boyle

bmoboyle@caltech.edu

Typhoon FLA 9000 gel imager

Siobhan MacArdle

smacardl@caltech.edu

Inverted fluorescence microscope (Nikon TS100)

Kun Miao

kmiao@caltech.edu

Upright dissection microscope (Nikon SMZ1000) + Clean bench

John Thompson

jthompso@caltech.edu

Affinity ITC

Stefan Petrovic

spetrovi@caltech.edu

Mammalian cell incubator

Kun Miao

kmiao@caltech.edu

BioRad qPCR (CFX 96 RT PCR)

Wen Zhou

wzhou@caltech.edu

4°C refrigerator

Alice Hsu

ahhsu@caltech.edu

FlexStation microplate reader

Stephen Grant

sgrant@caltech.edu

Beckman Avanti J-26 floor centrifuge

Alex Siegel

asiegel@caltech.edu

-80°C freezer

Brendan O'Boyle

bmoboyle@caltech.edu

Nanodrop 2000

Dan Zhou

dzhou@caltech.edu

Li-Cor IR imager

Chien-I Yang

cyang3@caltech.edu

Beckman Scintillation Counter

Rebekah Silva

rsilva@caltech.edu

Shaking incubator for bacteria

Terry Kim

tdkim@caltech.edu

Ultrospec cell density meter

John Thompson

jthompso@caltech.edu

QIAcube

Xun Wang 

Xwang2@caltech.edu

Neon transfection system

Gracie Zhang

gzhang@caltech.edu

Ice maker

Trixia Buscagan

tbuscaga@caltech.edu

Flexstation 3

Microplate reader with 5 read modes (including absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence) with additional robot functionality (8-channel pipettor) to add liquid reagents.

Contact: Stephen Grant, sgrant@caltech.edu

cellsig_flexstation.jpg

Odyssey Li-Cor IR Imager

Quantitative scanning of western blots at 700 and 800 nm.

Contact: Chien-I Yang, cyang3@caltech.edu

cellsig_odyssey.jpg

CFX 96 Real-Time PCR Detection System

Built upon the Bio-Rad C1000 96 well Thermal Cycler, utilizes filtered LEDs for excitation and is capable of monitoring 450-750 nM excitation/emission wavelength range. This system allows for simultaneous detection of up to 5 different fluorophores per reaction and is sensitive enough to detect one copy of target sequence in human genomic DNA.

Contact: Wen Zhou, wzhou@caltech.edu

cellsig_cfxrt.jpg

Nanodrop 2000

Micro-volume full spectrum UV-Visible spectrophotometer for sample volumes as low as 0.5 µL.

Contact: Dan Zhou, dzhou@caltech.edu

Nanodrop 2000

QIAcube

Fully automated sample preparation of Qiagen spin-column kits (up to 12 samples per run) for purification of DNA, RNA or proteins.

Contact: Xun Wang, xwang2@caltech.edu

QIAcube

I2500 KC Incubator Shaker

Floor-mounted shaker accepts up to 6-L flasks for culture.

Contact: Terry Kim, tdkim@caltech.edu

I2500 KC Incubator Shaker

Innova 43 Incubator Shaker

Floor-mounted temperature-controlled shaker accepts up to 6-L flasks for culture. Can go down to 5°C.

Contact: Terry Kim, tdkim@caltech.edu

Innova 43 Incubator Shaker

Avanti J-26 Centrifuge

Centrifuge up to 6 1-Liter cultures.

Contact: Alex Siegel, asiegel@caltech.edu

Avanti J-26 Centrifuge

Ultrospec 10

Portable spectrophotometer for OD600 readings.

Contact: John Thompson, jthompso@caltech.edu

Ultrospec 10

-80°C freezer

Intended for use as a back-up of individual lab freezers.

Contact: Brendan O'Boyle, bmoboyle@caltech.edu

-80°C freezer

Typhoon FLA 9000

High-resolution biomolecular imager capable of detecting storage phosphor, chemiluminescence, and multiplex fluorescence with 473 nm, 532 nm, and 625 nm excitation. Analysis done with ImageQuant software.

Contact: Siobhan MacArdle, smacardl@caltech.edu

Typhoon FLA 9000

Amaxa 4D-Nucleofector

Efficient electroporation of mammalian cells for transfection.

Contact: John Thompson (Hsieh-Wilson), x8119, jthompso@caltech.edu

Amaxa 4D-Nucleofector

Neon Transfection System

High efficiency electroporation transfection device for primary cells, stem cells, and difficult-to-transfect cells. It is capable of transfecting 10 or 100 µL volumes.

Contact: Alison E Ondrus/Ondrus Lab, aondrus@caltech.edu

Neon Transfection System

Nikon SMZ1000 Microscope

Used for dissections and imaging (transmitted light) with the camera attached. Zoom from 0.8 to 8X, in a clean bench to provide a sterile dissection/preparation work space.

Contact: John Thompson, jthompso@caltech.edu

Nikon SMZ1000 Microscope

Nikon TS100 Microscope

Used for basic visualization of cultured cells at 10 and 20X, allowing for monitoring and counting cells.

Contact: Kun Mmiao, kmiao@caltech.edu

Nikon TS100 Microscope

Clean Bench

Provides sterile environment for sensitive dissections or microscope work.

Contact: John Thompson (Hsieh-Wilson), x8119, jthompso@caltech.edu

Clean Bench

Laminar Flow Hoods/Biosafety Cabinets

Provides sterile environment for mammalian cell work.

Contact: Alice Hsu, ahhsu@caltech.edu

Laminar Flow Hoods

Mammalian Tissue Culture CO2 Incubator

Provides a controlled CO2 and temperature environment for cells during dissection, transfection or passaging done at the Center. It is intended for temporary use, and not long term housing of cultured cells.

Contact: Kun Miao, kmiao@caltech.edu

Eppendorf Centrifuge 5702

Tabletop centrifuge that can accomodate 15 or 50 mL conical tubes.

Contact: Dina Malounda, malounda@caltech.edu

Eppendorf Centrifuge 5702

Beckman LS 6000SC Scintillation Counter

The scintillation counter is designed to provide highly accurate, automated counting of the level of radioactivity in radioactivity-tagged samples.  Instrument used to count radioactive samples.  Note:  New users must complete Radioactivity Safety Training with Haick Issaian (haick.issian@caltech.edu), the Institute Radiation Safety Officer, before getting access to the scintillation counte.

Contact: Rebekah Silva (Barton), x3202, rsilva@caltech.edu

thumbnail_Scintillation Counter.jpg

Mailing address

Caltech Center for Molecular Medicine
Division of Chemistry and Chemical Engineering
MC 164-30
California Institute of Technology
Pasadena, CA 91125
USA